Abstract. The early promoter of the simian virus 40 (SV40) has been used as a model eukaryotic promoter for the study of DNA sequence elements and

Plasmid pMLS-SV40-EGFP from Dr. Jonathan Weissman's lab contains the insert GFP and is published in Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.

A small segment of the bovine albumin 5’flanking region, from -170 to +20, is sufficient for promoter activity and specificity [1]. The functional elements of the vector are the pUC origin of replication (pUC ori) for propagation in E.coli, the matrix attached region from the 5′-region of the human Interferone β-gene (S/MAR), the promoter of the bacterial ampicillin resistence gene for selection in E.coli (pAmp), the SV40 early promoter for selection in CHO cells (pSV40 The Vλ1 promoter activity was close to that of SV40 promoter/enhancer in most of the cell lines tested. We conclude that CMV and RSV promoter/enhancers contain stronger regulatory elements than do the SV40 and Vλ1 for expression of genes in lymphoid cell lines. SV40 and α-actin promoters were similar in their expression in muscle tissue, with levels about two-thirds less than that of the CMV promoter. The PgK promoter was the weakest of all five, and gave expression only about one-tenth of the CMV promoter in muscle. Linear Hygromycin Marker is a short ds DNA fragment consisting of the hygromycin resistance gene regulated by the SV40 promoter and ending with the SV40 polyadenylation signal. The 2.1-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a

Sv40 promoter

JCI - Renal tubule Cpt1a overexpression protects from RECIPES (8 days ago) This vector contains the phosphoglycerate kinase (PGK) promoter followed by an ATG start codon and an FRT recombination site, a splice acceptor-double polyA cassette, the tetracycline operator with a CMV minimal promoter, and an SV40 polyA signal, assembled in Bluescript. Se hela listan på academic.oup.com Millipore sv40 promoter Sv40 Promoter, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more CMV is typically a "stronger" promoter and recruits more transcriptional machinery at a greater rate than T7, it is also eukaryotes. There is a good summary of many different promoters available here: At pmirGLO Vector, SV40 early enhancer/promotor is located at 426–844 nucleotides, with a length of 419 nucleotides. It initiates the transcription of the hRluc-neo fusion protein coding region.

1320.

Plasmid pMLS-SV40-EGFP from Dr. Jonathan Weissman's lab contains the insert GFP and is published in Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.

Constitutive : Widespread expression, but may vary by cell type. SV40 promoter is also fairly strong, though generally somewhat weaker than EF1A and CAGG.

10 Nov 2020 The unleashing of the bidirectionality of the SV40 promoter activity and a breach of the transcription termination sequence required for the

240. 250. 260 SV40 polya. 1310. 1320.

transcribe SV40-like promoters suggested that there might be sequences within the SV40 promoter region that served as the target for Spi action. We set out to identify these sequences by analyzing the behavior of SV40 promoter mutants lacking portions of the upstream control se-quences.
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The underlined regions at the 5´ end of the fragment containing the SV40 promoter are the two 72 bp enhancer repeats. Se hela listan på en.wikipedia.org Recently, via microinjection into the cytoplasm and in situ hybridizations into a few cell types, it was shown that a region of Simian virus 40(SV40), specifically a c. 372-bp fragment of SV40 genomic DNA encompassing the SV40 promoter-enhancer-origin of replication (SV40 DTS), could enable the nuclear import of a plasmid carrying these sequences (Dean D.A. Exp. Cell Res. 230 (1997) 293). PSF-SV40 - SV40 PROMOTER PLASMID contains the Simian Virus 40 promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells.

GA-ori-00009.1) for high-level expression and replication in cell lines expressing the large T antigen (e.g.
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Poly (A) signals of both mammalian (SV40) and Drosophila (Mtn) origin efficiently directed stable RNA synthesis in S2 cells, and, as in mammalian cells, the SV40 late poly (A) signal was more efficient than the SV40 early poly (A) signal. Thus the process of polyadenylation appears to be conserved between mammalian and Drosophila cells.

SV40 promoter and SV40 origin of replication 28–366 β-globin intron 395–967 T7 promoter 1022–1040 EcoR I 1043 BamH I 1049 Bgl II 1055 SV40 polyA signal 1069–1202 pUC origin of replication 1342–2009 ampicillin resistance (bla) ORF 2160–3017 f1 origin of ss-DNA replication 3587–3893 PSF-SV40 - SV40 PROMOTER PLASMID contains the Simian Virus 40 promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells. Transcription termination is mediated by the SV40 poly-adenylation signals downstream of the MCS. Application Cloning in a gene SV40 basal promoter-LacZ Sequences (1) Addgene Sequences: Partial (1) Partial Sequences from Addgene (1) Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence. SV40 Promoter SV40 late poly(A) signal (for luc+ reporter) HpaI 2094 Synthetic poly(A) signal / transcriptional pause site (for background reduction) 0747V A08_4A Figure 4.

SV40, polyoma och adenovirus • Transformation- virusDNA integreras i värdcellens genom • Early genes stimul. Cell-proliferation • Binder

Two bcl-2 transgenic NOD Cell type and context-specific function of PLAG1 for IGF2 P3 promoter activity. construct with and without the H19 ICR and/or an SV40 enhancer, we confirm keratinocytes and for an NGAL promoter fragment transfected into A549 cells. sequence of the NGAL promoter against the heterologous SV40 promoter. selectively activates in vitro transcription from the SV40 promoter and binds to promoters by competing with Sp1 for binding to an overlapping binding motif.

doi: 10.1016/j.cell.2013.06.044. This plasmid is available through Addgene. transcribe SV40-like promoters suggested that there might be sequences within the SV40 promoter region that served as the target for Spi action. We set out to identify these sequences by analyzing the behavior of SV40 promoter mutants lacking portions of the upstream control se-quences.